Regeneration Status and Soil Nutrient Content in Burned Blue Pine Forest in Thimphu, Western Bhutan


  • Namgay Shacha Department of Forest and Park Services, Royal Government of Bhutan
  • Yonten Dorji Department of Forest Science, College of Natural Resources, Royal University of Bhutan
  • Arjun Nepal Mountain Hazelnut Ventures Private Limited, Mongar, Bhutan
  • Sangay Choden Department of Environment and Climate Studies, College of Natural Resources, Royal University of Bhutan
  • Tez Bdr Ghally Department of Forest and Park Services, Royal Government of Bhutan
  • Karma Chorten Dendup Department of Forest and Park Services, Royal Government of Bhutan



Blue Pine, Diversity, Dominance, Forest Fire, Regeneration


A forest fire and human disturbances are a major threat to regeneration and forest health. This study was conducted in western Bhutan in Thimphu, above Depsi. The Objective of the research was to assess diversity, species richness, regeneration, and soil nutrients content (N, P& K) along the altitudinal gradient after a forest fire. A total of 30 standard plots of size 20x20 m plots were established in the burned blue pine forest systematically, along the altitudinal gradient. Systematic random sampling was adopted. Sampling was carried out at three different altitudinal zones that is; lower (2500-2700 masl), middle (2701-2900 masl), and higher (2901-3100 masl) altitude. A total of 1703 individual plants belonging to 21 families were recorded from the study area. The diversity, regeneration, and soil nutrient increased till mid-altitude and decreased with further increase in altitude, making a unimodal, hump shape pattern. The regeneration, soil nutrient, and diversity were higher in the gentle slope and the slope facing southeast due to the longer duration of sunlight exposition. The regeneration and diversity of vegetation showed a positive correlation with aspect, carbon, phosphorous, and nitrogen (p<.05). The diversity and regeneration after a fire was affected by environmental factors, especially soil nutrients, aspect, and slope. The study forms the baseline data for the forest manager to sustainably restore the forest after a fire.  Species resistant to fire can be chosen for forest restoration. It can also be incorporated in management plans for sustainable management and conservation of mountain blue pine forests.


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Acharya, A. S., Prakash, A., Saxena, P., & Nigam, A. (2013). Sampling: Why and how of

it. Indian Journal of Medical Specialties, 4(2), 330-333.

Ashtamoorthy, S. (2014). A modified Sorensen's index to compare similarity between plant communities. <>. Accessed 13 November 2018.

Attri, V., Sharma, D. P., & Dhiman, R. (2017). Floristic Diversity and Natural Regeneration Status of Chir pine (Pinus roxburghii Sargent) Forest: a case study of Rajgarh Forest Division of Himachal Pradesh. Bull Environ Pharm Life Sci, 6, 1-6.

Bari, F., Wood, M. K., & Murray, L. (1995). Livestock grazing impacts on interrill erosion in Pakistan. Rangeland Ecology & Management/Journal of Range Management Archives, 48(3), 251-257.

Bhadra, A. K., Dhal, N. K., & Pattanayak, S. K. (2014). Altitude based tree species occurrence in the protected natural forest of Gandhamardan Hill ranges, Balangir, Odisha. Biolife, 2(2), 420-441.

Bhat, G. M., Mughal, A. H., Malik, A. R., Khan, P. A., & Shazmeen, Q. A. S. B. A. (2015). Natural regeneration status of blue pine (Pinus wallichiana) in North West Himalayas, India. The Ecoscan, 9(3&4), 1023-6.

Bremner, J. M. (1960). Determination of nitrogen in soil by the Kjeldahl method. The Journal of Agricultural Science, 55(1), 11-33.

Brown, G.W. (2018). The impact of timber harvest on soil and water resources, Oregon State University and the United States department of agriculture: Acts of congress.

Certini, G., Nocentini, C., Knicker, H., Arfaioli, P., & Rumpel, C. (2011). Wildfire effects on soil organic matter quantity and quality in two fire-prone Mediterranean pine forests. Geoderma, 167, 148-155.

Dangwal, L.R. & Tajinder, S. (2012). Comparative vegetational analysis and Pinus roxburghii Sarg regeneration in relation to their disturbances in some Chir pine forest of block Nowshera, district Rajouri, J and K, India. ISCA Journal of Biological Sciences, 1(1): 47-54.

Day, A. D., & Ludeke, K. L. (1993). Phosphorus as a plant nutrient. In Plant nutrients in desert environments (pp. 45-48). Springer, Berlin, Heidelberg.

Frondorf, E. (2018). Are oak and pine trees regenerating after fires in the park, Shenandoah National Park Trust. 1-8.

Gempa. (2014). Growth performance of blue pine forest (Pinus wallichiana) at different altitudinal zones in northern and southern aspect of Chendebji, Trongsa. College of Natural Resources: pp 1- 30.

Gracia, M., Retana, J., & Roig, P. (2002). Mid-term successional patterns after fire of mixed pine–oak forests in NE Spain. Acta Oecologica, 23(6), 405-411.

Gyeltshen, C., Gratzer, G., Meigs, G., & Keeton, W. (2016). Fire risks in blue pine forests of

Bhutan (Doctoral dissertation, Master thesis, University of Natural Resources and Life Sciences (Boku), Vienna, Austria).

Konsam, B., Phartyal, S. S., Kumar, M., & Todaria, N. P. (2017). Life after fire for Understory plant community in subtropical Chir pine forest of Garhwal Himalaya. Indian Forester, 143(8), 759-766.

Kuensel. (2016). Preventing forest fire. <>. Accessed 11 November 2018.

Kutiel, P., & Naveh, Z. (1987). The effect of fire on nutrients in a pine forest soil. Plant and Soil, 104(2), 269-274.

Maia, P., Keizer, J., Vasques, A., Abrantes, N., Roxo, L., Fernandes, P., & Moreira, F. (2014). Post-fire plant diversity and abundance in pine and eucalypt stands in Portugal: Effects of biogeography, topography, forest type and post-fire management. Forest ecology and management, 334, 154-162.

Moktan. D. & Tenzin, U. (2016). Field Labotory guide. College of Natural Resources. Punakha: pp. 1- 65.

Mukhia, P. K., Wangyal, J. T., & Gurung, D. B. (2011). Floristic composition and species diversity of the chirpine forest ecosystem, Lobesa, Western Bhutan. For. Nepal, 1-4.

Neeman, G. (2014, December). Regeneration of natural pine forest – review of work done after the 1989. International journal of wildland fire. 7(4): 306-295.

National Forest Inventory (NFI) (2016). National forest inventory report: stock taking nation’s forest resources. Thimphu, Bhutan: Department of forest and park services.

Nima. (2018). Fire destroyed about 16000 acres of forest reserve in 2017-2018. <>. Accessed 1 November 2018.

National Statistics bureau (NSB). (2016). Statistical yearbook of Bhutan 2016.<>publications>. Accessed 22 October 2018.

Ogwu, M. C., Osawaru, M. E., & Obayuwana, O. K. (2016). Diversity and Abundance of Tree Species in the University of Benin, Benin City, Nigeria. Applied Tropical Agriculture, 21(3), 46-54.

Pem, D. (2016). Forest fire in Thimphu in a day. <>. Accessed 12 October 2018.

Parashar, A., & Biswas, S. (2003). The impact of forest fire on forest biodiversity in the Indian Himalayas (Uttaranchal). In XII World Forestry Congress (Vol. 358).

Rawat, B., Gairola, S., Sekar, K. C., & Rawal, R. S. (2014). Community structure, regeneration potential and future dynamics of natural forest site in part of Nanda Devi Biosphere Reserve, Uttarakhand, India. African Journal of Plant Science, 8(7), 380-391.

Sahu, S. C., Pani, A. K., Mohanta, M. R., & Kumar, J. (2019). Tree species diversity, distribution and soil nutrient status along altitudinal gradients in Saptasajya hill range, Eastern Ghats, India. Taiwania, 64(1), 28.

Sankey, J. B., Kreitler, J., Hawbaker, T. J., McVay, J. L., Miller, M. E., Mueller, E. R., ... &

Sankey, T. T. (2017). Climate, wildfire, and erosion ensemble foretells more sediment in western USA watersheds. Geophysical Research Letters, 44(17), 8884-8892.

Schreuder, H.T., Gregoire, T.G. & Wood, G.B. (1993). Sampling methods for multi-resource forest inventory, USA: Wiley.1-464.

Shannon, C.E. (1948). A Mathematical Theory of Communication. Bell System Technical Journal, 27(4).

Sharma, N. &. Raina. A. K. (2013). Composition, structure and diversity of tree species along an altitudinal gradient in Jammu province of north_western Himalayas, India. Journal of Biodiversity and Ecological Science.

Subedi, C. K., Gurung, J., Ghimire, S. K., Chettri, N., Pasakhala, B., Bhandari, P., & Chaudhary, R. P. (2018). Variation in structure and composition of two pine forests in Kailash Sacred Landscape, Nepal. Banko Janakari, 28(1), 26-36.

Tshewang, P. (2015). Regeneration and stand structure of pinus wallichina along elevation gragient at Chugphel Tangsibbe, Bumthang, (B.Sc thesis). College of Natural Resources, Lobesa. 1-65.

Turrion, M. B., Lafuente, F., Aroca, M. J., López, O., Mulas, R., & Ruipérez, C. (2010). Characterization of soil phosphorus in a fire-affected forest Cambisol by chemical extractions and 31P-NMR spectroscopy analysis. Science of the Total Environment, 408(16), 3342-3348.

Vega, G. D., De las Heras, J., & Moya, D. (2018). Post-fire regeneration and diversity response to burn severity in Pinus halepensis Mill. forests. Forests, 9(6), 299.

Verma, S., Singh, D., Mani, S., & Jayakumar, S. (2017). Effect of forest fire on tree diversity and regeneration potential in a tropical dry deciduous forest of Mudumalai Tiger Reserve, Western Ghats, India. Ecological Processes, 6(1), 1-8.

Wangdi, W. J. (2002). Cattle grazing in the conifer forests of Bhutan. Bio one: 1-8.

Watanabe, F. S., & Olsen, S. R. (1965). Test of an ascorbic acid method for determining phosphorus in water and NaHCO3 extracts from soil. Soil Science Society of America Journal, 29(6), 677-678.

Prober, S., & Wiehl, G. (2011). Relationships among soil fertility, native plant diversity and exotic plant abundance inform restoration of forb-rich eucalypt woodlands. DOI:10.1111/j.1472-4642.2011.00872.x.

Xu, M., Ma, L., Jia, Y., & Liu, M. (2017). Integrating the effects of latitude and altitude on the spatial differentiation of plant community diversity in a mountainous ecosystem in China. PloS one, 12(3).

Xue, R., Yang, Q., Miao, F., Wang, X., & Shen, Y. (2018). Slope aspect influences plant biomass, soil properties and microbial composition in alpine meadow on the Qinghai-Tibetan plateau. Journal of soil science and plant nutrition, 18(1), 1-12.

Zapata, F. A., Gaston, K. J., & Chown, S. L. (2003). Mid‐domain models of species richness Gradients: assumptions, methods and evidence. Journal of Animal Ecology, 72(4), 677-690.

Zeng, X. H., Zhang, W. J., Song, Y. G., & Shen, H. T. (2014). Slope aspect and slope position have effects on plant diversity and spatial distribution in the hilly region of Mount Taihang, North China. J. Food Agric. Environ., 12, 391-397.

Zhang, Y., Li, C., & Wang, M. (2019). Linkages of C: N: P stoichiometry between soil and leaf and their response to climatic factors along altitudinal gradients. Journal of Soils and Sediments, 19(4), 1820-1829.



How to Cite

Namgay Shacha, Yonten Dorji, Nepal, A., Sangay Choden, Tez Bdr Ghally, & Karma Chorten Dendup. (2021). Regeneration Status and Soil Nutrient Content in Burned Blue Pine Forest in Thimphu, Western Bhutan. Indonesian Journal of Social and Environmental Issues (IJSEI), 2(1), 48-58.




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