Biostimulatory Influence of Biochar on Degradation of Petroleum Hydrocarbon Impacted Soil
Keywords:Bacteria Population, Biochar, Bone Char, Total Petroleum Hydrocarbon, Wood Char
Soil pollution caused by petroleum hydrocarbon and its derivatives has become a grave global issue. Physico-chemical techniques are often expensive. However, bioremediation of petroleum hydrocarbon polluted soil is cost-effective. Therefore, the study was carried out to assess the biostimulatory influence of biochar on the degradation of petroleum hydrocarbon impacted soil in NNPC Depot, kano state. Soil samples were randomly collected from the polluted site to obtain a composite sample. About 400 g of the polluted soil was filled into pots and arranged in a 2x2 factorial experiment in a completely randomized design with three replications. Bone and wood char was at 2 levels (0 and 50 g/pot) each. Data were collected on the physicochemical properties (pH, TN, and Av. P) of the soil, Total Petroleum Hydrocarbon (TPH), and bacterial population. Data were analyzed using ANOVA at α0. 05. Results obtained from the study show that biochar application significantly (p<0.05) enhanced TPH degradation and bacterial population in the polluted soil. However, Bone char significantly(p<0.05) enhanced TPH degradation and bacterial population the most compared to wood char. Combined bone and wood char application resulted in significantly (p<0.05) lower residual TPH content in the polluted soil compared to using bone or wood char alone. Thus, bone and wood char should be used in the bioremediation of petroleum hydrocarbon impacted soils.
Abioye, O. P.; Agamuthu, P. & Abdul-Aziz, R.A. (2012). Biodegradation of Used Motor Oil using Organic Waste Amendment. Hindawi Publishing Corporation. Biotechnology Research
Adeleye, A.O. & Yerima, M.B. (2019). Isolation and Identification of Spent Engine Oil Utilizing Bacteria from Mechanic Village Dutse, Jigawa State. Paper presented at the 42nd Conference of the Nigerian Society for Microbiology. Crawford University, Igbesa, Ogun State.
Adeleye, A. O.; Yerima, M. B., Nkereuwem, M. E., Onokebhagbe, V. O., Shiaka, P. G., Amoo, F. K. & Adam, I. K. (2019). Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil. Novel Research in Microbiology Journal. 3(5): 471-484.
Adeleye, A. O., Nkereuwem, M. E., Omokhudu, G. I., Amoo, A. O., Shiaka, G. P. & Yerima, M. B. (2018). Effect of Microorganism in the Bioremediation of Spent Engine Oil and Petroleum Related Environmental Pollution. J. Appl. Sci. Environ. Manage. 22(2): 157-167.
Agarry, S. E; Aremu, M. O; & Aworanti, O. A. (2013a). Kinetic modelling and half-life study on bioremediation of soil co-contaminated with lubricating motor oil and lead using different bioremediation strategies. Soil and Sediment Contam. - An Int. J. 22 (7): 800-816.
Agarry, S. E; Aremu, M. O; & Aworanti, O. A. (2013b). Kinetic modelling and half-life study on enhanced soil bioremediation of bonny light crude oil amended with crop and animal-derived organic wastes. J. Pet Environ. Biotechnol. 4: 137.
Ali, N., Dashti, N., Khanafer, M., Al-Awadhi, H. & Radwan, S. (2020). Bioremediation of soils saturated with spilled crude oil, Sci Rep. 10 (1):1116.
Alvarez, P. J. J. & Vogel, T. M. (2011). Substrate interactions of benzene, toluene, and para-xylene during microbial degradation by pure cultures and mixed culture aquifer slurries. Applied and Environmental Microbiology. 57 (10): 2981-2985.
Atlas, R. M. & Bartha, R. (1992). Microbial Ecology: Fundamentals and Applications, Benjamin/Cumming Publishing Company, Menlo Park, Calif, USA.
Barrow, G. I. & Feltham, R. K. A. (1993). Cowan and Steel's Manual for the Identification of Medical Bacteria. Cambridge University Press 3rd Edn Pp. 52-101.
Beesley, L., Moreno-Jiménez, E. & Gomez-Eyles, J. L. (2010). Effects of biochar and green waste compost amendments on mobility, bioavailability and toxicity of inorganic and organic contaminants in a multi-element polluted soil. Environmental Pollution,
Bento, F. M., Camargo, F. A. O., Okeke, B. C. & Frankenberger Jr., B. T. (2005). Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresource Technology. 96 (9): 1049-1055.
Beolchini, F., Rocchetti, L., Regoli, F. & Dell’Anno, A. (2010). Bioremediation of marine sediments contaminated by hydrocarbons: experimental analysis and kinetic modelling,” Journal of Hazardous Materials. 182 (1–3): 403-407.
Bijay, T., Ajay, K. K. C. & Anish, G. (2012). A review on bioremediation of petroleum hydrocarbon contaminants in soil. Kathmandu University Journal of Science, Engineering and Technology. 8 (I):164-170.
Bouyoucos, C. H. (1951). A recalibration of hydrometer method for making mechanical analysis of soils. Agronomy Journal. 43 (9): 434-438.
Bray, R. H. & Kurtz, L.T. (1945). Determination of Total Organic and Available Forms of Phosphorus in Soils. Soil Science. (59): 39-45.
Chan, K.Y. & Xu, Z. (2009). Biochar: nutrient properties and their enhancement. In: Lehmann J., Joseph S. (Eds.), Biochar for Environmental Management: Science and Technology. Earthscan, London.
Dadrasnia, A.; Salmah, I.; Emenike, C.U. & Shahsavari, N. (2015). Remediation of oil contaminated media using organic material supplementation. Petroleum Science and Technology. (33): 1030-1037.
Erdogan, E. E. & Karaca, A. (2011). Bioremediation of crude oil polluted soils. Asian Journal of Biotechnology. 3 (3): 206-213.
Frick, C. M., Farrell, R. E. & Germida J. J. (1999). Assessment of phytoremediation as an in-situ technique for cleaning oil-contaminated sites. Report submitted to Petroleum Technology Alliance of Canada.
Ijah, U. J. J. & Antai, S. P. (2003). Removal of Nigerian light crude oil in soil over a 12-month period. International Biodeterioration and Biodegradation. 51 (2): 93-99.
Ikiogha, D., Otaraku, I., Momoh, Y. & Welcome, M. (2019). Comparative effects of bone char and NPK agricultural fertilizers on hydrocarbon utilizing bacteria and fungi in crude oil polluted soil. Am. Scientific Res. J. Eng., Technol. Sci. (55): 35-49.
Kauppi, S., Sinkkonen, A. & Romantschuk, M. (2011). Enhancing bioremediation of diesel-fuel-contaminated soil in a boreal climate: comparison of biostimulation and bioaugmentation. International Biodeterioration and Biodegradation. 65 (2): 359-368.
Lehmann, J. (2009). Biological carbon sequestration must and can be a win win approaches. Climatic Change. (97): 459-463.
McLean, E. (1982). Soil pH and lime requirement. Methods of soil analysis. Part 2. Chemical and microbiological properties. 199 - 224.
Nelson, D.W. & Sommers, L.E. (1996). Total carbon, organic carbon and organic matter. In: Methods of Soil Analysis, Part 2, 2nd ed., A.L. Page et al., Ed. Agronomy. 9:961-1010. American Society of Agronomy, Inc. Madison, WI.
Nkereuwem, M. E., Fagbola, O., Okon, I. E., Adeleye, A. O. & Nzamouhe, M. (2020a). Bioremediation potential of mycorrhiza fungi in crude oil contaminated soil planted with Costus lucanusianus. Amazonian Journal of Plant Research. 4(1): 441-455.
Nkereuwem, M. E., Fagbola, O., Okon, I. E., Edem, I. D., Adeleye, A. O. & Onokebhagbe, V. O. (2020b). Influence of a mycorrhizal fungus and mineral fertilizer on the performance of Costus lucanusianus under crude oil contaminated soil. Novel Research in Microbiology Journal. 4(3): 808-824.
Nkereuwem, M. E., Edem, I. D. & Fagbola, O. (2010). Bioremediation of Oil-polluted soils with Organomineral Fertiliser (OMF) and Mexican Sunflower (Tithonia diversifolia). Nigerian Journal of Agriculture, Food and Environment. 6 (1 and 2): 13-20.
Novak, I., Kirkin, V., McEwan, D.G., Zhang, J., Wild, P., Rozenknop, A., Rogov, V., Löhr, F., Popovic, D. & Occhipinti, A. (2010). Nix is a selective autophagy receptor for mitochondrial clearance. EMBO Rep.11: 45-51.
Nwogu, T. P., Azubuike, C. C. & Ogugbue C. J. (2015). Enhanced Bioremediation of soil artificially contaminated with petroleum Hydrocarbons after amendment with Capra aegagrus hirus (Goat) manure. Biotechnology Research International. ID 657349.
Ochei, J. O & Kolhatkar, A. A. (2008). Medical Laboratory Science: Theory and Practice. Tata McGraw Publishing Company Limited.
Ofoegbu, R. U., Momoh, Y. O. L. & Nwaogazie, I. L. (2014). Bioremediation of Crude Oil Contaminated Soil Using Organic and Inorganic Fertilizers. Journal of Petroleum & Environmental Biotechnology. 6:1-6.
Onokebhagbe, O. V., Abdu, N. & Santuraki, H. A. (2018). Residual effects of biochar on yield of grain amaranths (Amaranthus cruentus L.) grown on Alfisols of Nigerian Northern Guinea and Sudan savanna agro-ecologies. Dutse Journal of Agriculture and Food Security, 5 (1): 97-108.
Onuoha, S. C. (2013). Stimulated biodegradation of spent lubricating motor oil in soil amended
with animal droppings. Journal of Natural Sciences Research. 3 (12): 106-116.
Park, J. H., Choppala, G. K., Bolan, N. S., Chung, J. W. & Chuasavathi, T. (2011). Biochar reduces the bioavailability and phytotoxicity of heavy metals. Plant Soil. (348): 439-451.
Udo, E.J. & Ogunwale, J.A. (1986). Laboratory Manual for Analysis of Soil, Plant and Water Samples. University Press Ibadan.
Ugwoha, E. & Iwuchukwu, O. L. (2020). Modeling the Bioremediation of Petrol Contaminated Soil by Clogged-Drainage Bacteria. Uniport Journal of Engineering and Scientific Research. 5 (SI):7 1-81.
U.S. EPA. (2003). Method 8015C (SW-846): Nonhalogenated Organics Using GC/FID, Revision 4. Washington, DC.
Wang, Tong-tong, M. A. Jiang-bo, QU Dong. (2017). Characteristics and mechanism of copper adsorption from aqueous solutions on biochar produced from sawdust and apple branch. Journal of Environmental Science. 38 (5): 2161-2171.
Xiao, X., Chen, B., Lizhong, Z. (2014). Transformation, morphology and dissolution of silicon and carbon in rice straw derived biochars under different pyrolytic temperatures. Environmental Science & Technology. (48): 3411-3419.
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